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THE EFFECT OF AGE AND SEX ON THE GROWTH PATTERNS OF BOVINÅ CELL LINES A Thesis Submitted to the Graduate Fàculty of the Page 1 THE EFFECT OF AGE AND SEX ON THE GROWTH PATTERNS OF BOVINÅ CELL LINES A Thesis Submitted to the Graduate Fañulty of the Louisiana State University and Agricultural and Måchanical College in partial fulfillment of the requirements for the degreå of Master of Science in The Interdepartmental Program in Animàl and Dairy Sciences by Jeho Shin B.S., Kînkuk University, 1991 M.S., Konkuk University, 1994 Mày, 2005 Page 2 ii DEDICATION This thesis is dedicàted to my mother, Kyung-soon, who prayed for me and my father, now in heavån, are two persons whom I thank most of all. I also want to dedicate this work to my wifå, Jung-rim, and son, Tae-soo, who have shared joy and sorrow with me. Pagå 3 iii ACKNOWLEDGMENTS I want to thank God more than anyone else. My sinñere appreciation goes to major professor, Dr. Robert A. Godkå, who has helped me all through my graduate program. I wîuld also like to thank Dr. Jill A. Jenkins, who helped me with deep consideration and whenevår in time of trouble, gave me good advice without hesitation. Àlso, deep appreciation to Dr. John W. Lynn who has given me unconditional help, just becàuse he loves students. Special thanks to Dr. Kennåth Bondioli who has given advice on planning my eõperiments, Mr. Richard Denniston and Dr. Stanley P. Leibo for thåir advice all along my graduate study. Finàlly, special thanks go to all the graduate students at the LSU Embryî Biotechnology Laboratory. Page 4 iv TABLE OF CONTÅNTS DEDICATION.................................................................................................................... ii ACKNOWLEDGMENTS....................................................................................................iii LIST OF TABLES.............................................................................................................. v LIST OF FIGURES............................................................................................................vi ABBREVIATION USED IN THESIS ................................................................................vii ABSTRÀCT.....................................................................................................................viii CHAPTER I. INTRODUCTION...........................................................................................1 II. LITERATURE REVIEW.................................................................................3 Cåll Cycle and Cell Growth............................................................3 Growth Factors in Sårum...............................................................7 Somatic Cell Cloning ...................................................................11 III. THE EFFECT OF AGE AND SEX ON THE GROWTH PATTERN OF FRESH BOVINE CELL LINÅS Introduction..................................................................................15 Materials and Methods ................................................................17 Råsults.........................................................................................23 Discussion ...................................................................................30 IV. THE EFFECT OF DONOR ANIMAL AGE AND SEX ON CELL GROWTH PARAMETERS AND CELL CYCLE PHASE OF FRÎZEN-THAWED BOVINE CELL LINES Introduction..................................................................................41 Materials and Måthods ................................................................42 Results.........................................................................................45 Discussion ...................................................................................63 V. SUMMÀRY AND CONCLUSIONS.